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The Organizing Committee of The 9th Asian International Association of Dental Traumatology –
Cebu (AADT Cebu) is calling for abstract submissions. To present your research at the
conference, your abstract must first be reviewed. You will receive a confirmation of
abstract acceptance within two weeks and requested to register. Upon registration,

your attendance at the conference will be confirmed. Bring a printout of your registration

confirmation to the venue to pay for the registration fee.


1. All abstracts must be written in English (Font: Times New Roman, 12 points).
2. Refer to the following:

                  Title (Font: Times New Roman, 14 points, Bold),
                  Name of Presenter (e-mail, other co-investigators, affiliation and address)
                  Your photo (about 3 X 4 cm),
                  Abstract (
Objective, Materials & Method, Results, Conclusion)

3. Word limit for Abstract is 300 words
4. Key words (3 – 5 words)
5. Brief CV (Educational background and Professional experience) Half-page
6. Specify mode of presentation (Oral presentation or Poster presentation)


NOTE: Your Title, Name, Affiliation, Abstract, Photo and Short CV should fit in one A4 page.

Please send your abstract to Omar Rodis: omarodis@tokushima-u.ac.jp

Low level fluoride stimulates epithelial-mesenchymal interaction in oral mucosa
Ujjal K. Bhawal
Assistant Professor, Department of Biochemistry and Molecular Biology,
Nihon University School of Dentistry at Matsudo, Japan

Objectives: Oral mucosa consists of the superficial epithelium and the underlying
lamina propria, and it functions as a barrier against exogenous substances. In
development, interactions of stem/progenitor cells of the epithelium and mesenchyme
are crucial to the morphogenesis of oral mucosa. Our previous work in low level
fluoride induced cell motility of epithelial cells has yielded important clues for
periodontal physiology. This study focuses on a working concept of low level fluoride
to provide a conducive oral environment for pivotal epithelial mesenchymal


Materials and methods: Cultured human primary gingival epithelial cells
treated with 50 μM NaF were investigated by DNA microarray. Quantitative real-time
PCR and an in vivo experimental rat skin wound model were employed to confirm the
findings obtained with the microarray analysis.

Results: Microarray data revealed that low level fluoride-treated human gingival
epithelial cells elevated various biological processes. The key proliferation markers,
FGF2 and FGF7, and mesenchymal marker, Twist1, expression was up-regulated and
quantitative real-time PCR confirmed this observation. Our in vivo study revealed that
low concentration of NaF increased FGF2, FGF7, and Twist1 protein expression in
fluoride-treated skin wound tissues compared with controls.

Conclusion: These results provide new information on low level fluoride-induced
epithelial-mesenchymal interactions and may thus aid in the understanding of oral
mucosa development.

Key words: Low level NaF, Human gingival epithelial cell, FGF2, FGF7, Twist1

Brief CV
Educational Background

1987-1993                 Dhaka Dental College, Bangladesh 

1997-2001                 Hiroshima University Graduate School of Dentistry, Japan

                                   (Ph.D. from Hiroshima University)

Professional experience
2004-2006               Research Fellow, Department of Biochemistry, Hiroshima University  
School of Dentistry, Japan

2006-present          Researcher, Department of Molecular Pathology, Nara Medical University, Japan

2006-2011                Research Fellow, Kanagawa Dental University, Japan
2011-present            Visiting Lecturer, Kanagawa Dental University, Japan
2011-present            Assistant Professor, Department of Biochemistry and Molecular Biology,

                                   Nihon University School of Dentistry at Matsudo, Japan